DNA Sequencing and Fragment Analysis

The DNA sequencing of the human genome was the greatest task in history of analytical chemistry.DNA Sequencing Fragment Analysis services using Applied Biosystems instrumentation and chemistries. Four tools were required for this task. First, the basis for virtually all DNA sequencing was the dideoxy-chain terminating reaction, developed by Sanger. Most large-scale DNA sequencing facilities use to label and detect the four bases, and capillary to separate DNA molecules on the basis of size so that the base located at each position in the sequence can be identified.

DNA is a polymer comprised of four building blocks named A, C, G, and T, depending on the specific biochemical composition. DNA sequencing is the method by which the order of nucleotides in a segment of DNA is determined. In this method, the DNA that is to be sequences, called the template, is hybridized with a short, complementary oligonucleotide, called the primer. DNA polymerase and deoxynucleotides are added to the mixture, and the enzyme catalyzes the synthesis of the complementary DNA strand, starting at the primer and extending in the 3' direction. Our DNA sequencing facility focuses on Applied Biosystems chemistries and equipment, as we have found that this combination allows us to consistently.

In contrast, the public DNA sequencing effort employed a directed strategy that prepared an
intermediate set of very large DNA molecules, which were cloned in bacteria. These bacterial
artificial chromosomes were mapped onto the human genome, so that their location was precisely known. For a recent review of DNA sequencing technology development, see the
following feature article in Genomics and Proteomics "DNA sequencing ".